GSM7083704: RPE-KO CK2 [0414]; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CSNK2A1
Cell type
Cell type Class
Neural
Cell type
RPE
NA
NA
Attributes by original data submitter
Sample
source_name
RPE-CK2 deficient
cell line
RPE-CK2 deficient
cell type
Retinal Pigment Epithelial
chip antibody
CK2 (Abcam, ab#70774)
treatment
Early G1
geo_loc_name
missing
collection_date
missing
Sequenced DNA Library
library_name
GSM7083704
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 min for PolII, H3K4me3, H3K27ac, or PolIISer2P ChIP, and with di-succinimidyly-glutarate for 45 min, followed by 10 min fixation with 1% formaldehyde for CK2a ChIP. Fixation was performed at room temperature and terminated by incubation with 125 mM glycine for 10 min. Fixed chromatin was sonicated in lysis buffer containing 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1% Triton X-100, 1% NP-40, 1% sodium cholate, 0.1% SDS, using a Branson Sonifier 250D to generate DNA fragments with a mean length of 300-1000 bp. Input and ChIP DNA was processed and sequenced according to the manufacturer's instructions. Briefly, DNA was sheared to an average size of ~150 bp with ultrasonication (Covaris), end-repaired, ligated to sequencing adapters, amplified, size-selected, and DNA was then sequenced to generate single-end 65-bp reads using the Illumina HiSeq-2500 systems.